G測(cè)序數(shù)據(jù)
個(gè)樣本數(shù)據(jù)
篇研究相關(guān)文獻(xiàn)
篇影響因子10分以上文獻(xiàn)
共表達(dá)分析中,整合大量相關(guān)公共樣本測(cè)序數(shù)據(jù),可構(gòu)建出相較于常規(guī)樣本量下可靠度更高的基因共表達(dá)網(wǎng)絡(luò),從而基于該網(wǎng)絡(luò)進(jìn)行更加準(zhǔn)確的后續(xù)分析:a)預(yù)測(cè)目標(biāo)轉(zhuǎn)錄因子的下游調(diào)控基因、目標(biāo)調(diào)控網(wǎng)絡(luò)中的關(guān)鍵轉(zhuǎn)錄因子;b)預(yù)測(cè)ncRNA與mRNA之間的靶向關(guān)系;c)基于網(wǎng)絡(luò)中已知功能基因推測(cè)同網(wǎng)絡(luò)中其他功能未知基因功能;d) 將每個(gè)共表達(dá)模塊分別作為一個(gè)整體,計(jì)算其與各組織或各發(fā)育時(shí)間點(diǎn)之間的相關(guān)性,建立各組織相關(guān)或各時(shí)期相關(guān)基因表達(dá)網(wǎng)絡(luò)……
[1] Wisecaver JH et al. A Global Coexpression Network Approach for Connecting Genes to Specialized Metabolic Pathways in Plants. Plant Cell. 2017
[2]?Wen Z et al.Expression profiling and functional annotation of noncoding genes across 11 distinct organs in rat development.?Sci Rep.?2016
研究某一類(lèi)基因的轉(zhuǎn)錄水平在不同處理下(或不同組織部位間、或不同發(fā)育時(shí)間點(diǎn)等)的變化規(guī)律,可整合多個(gè)類(lèi)似研究中的公共測(cè)序數(shù)據(jù)來(lái)共同揭示該變化規(guī)律,使結(jié)果更加可靠。
[1] Patil G. et al. Soybean (Glycine max) SWEET gene family: insights through comparative genomics, transcriptome profiling and whole genome re-sequence analysis. BMC Genomics. 2015
[2] Matthijs M et al.Profiling of the Early Nitrogen Stress Response in the Diatom Phaeodactylum tricornutum Reveals a Novel Family of RING-Domain Transcription Factors. Plant Physiol. 2016
1. 整合同一物種公共測(cè)序數(shù)據(jù),構(gòu)建物種完備轉(zhuǎn)錄本序列參考集,用于后續(xù)功能分析。
Iyer MK et al. The landscape of long noncoding RNAs in the human transcriptome. Nat Genet. 2015
2. mRNA-ncRNA聯(lián)合分析中,利用公共數(shù)據(jù)補(bǔ)充其中一種類(lèi)型的RNA測(cè)序數(shù)據(jù)。
Liu X et al. MicroRNA-mRNA regulatory networking fine-tunes the porcine muscle fiber type, muscular mitochondrial respiratory and metabolic enzyme activities. BMC Genomics. 2016
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條雞基因功能信息
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條雞基因功能信息